Diferenciación de carnes de jabalí europeo (Sus scrofa scrofa) y cerdo doméstico (Sus scrofa domestica) mediante el análisis por PCR de la región mitocondrial D-loop y el gen nuclear MC1R

Abstract

This work describes the differentiation of European wild boar (Sus scrofa scrofa) and domestic swine (Sus scrofa domestica) meats by PCR targeting sequences from the mitochondrial displacement loop (D-loop) region and the nuclear melanocortin receptor 1 (MC1R) gene. Detailed analysis of every D-loop sequence obtained indicated that PCR RFLP differentiation between wild and domestic Sus scrofa meats was not possible. Nevertheless, the PCR-RFLP technique developed targeting the MC1R gene generated characteristic PCR–RFLP profiles that allowed discrimination among meats from wild and domestic swine specimens using BspHI and BstUI endonucleases.