Evaluación de una técnica de RT-PCR específica para la detección del serotipo 8 del virus de la lengua azul en cabras
Abstract
Bluetongue virus (BTV) is an Orbivirus transmitted by haematophagous insects of the genus Culicoides. In the north of the Iberian Peninsula, BTV-8 has appeared recently, affecting sheep and cattle. Bluetongue serotypes are so different among them, that vaccines do not offer cross-protection. Thus, it is important to detect which serotype is affecting an animal, in order to apply the correct vaccine. An RT-PCR assay has been developed recently to detect BTV-8 in cattle and sheep. In this study we have evaluated this RT-PCR technique as a diagnostic tool for the specific detection of BTV- 8 in goats. Sera samples from 15 infected goats and supernatants from BTV-8 infected cell cultures were analyzed. No positive results were obtained for the analyzed samples.Downloads
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