Lung surfactant diminishes non-typeable "Haemophylus influenzae" invasion of pneumocytes
Abstract
Lung surfactant is a lipoprotein complex that lines the alveolar fluid and is the first barrier against respiratory pathogens. The pathogen non typeable Haemophilus influenzae (NTHi) causes persistent infections in patients of chronic obstructive pulmonary disease (COPD) due to its ability to be internalized and survive in pneumocytes. Our objective was to characterize the effect of the lipid component of pulmonary surfactant, with or without surfactant proteins SP-B and SP-C, in the invasion and adhesion of NTHi to pneumocytes.
We found that multilamellar vesicles of surfactant, inhibited invasion of pneumocytes by NTHi, due at least in part to lower adhesion to epithelial cells. Moreover, unilamellar vesicles surfactant, that are endocytosed by pneumocytes, inhibited internalization of NTHi, but only the presence of SP-B and SP-C reduced adhesion of NTHi to epithelial cells. Our results show that surfactant lipids and SP-B and SP-C inhibit NTHi invasion of pneumocytes through various mechanisms.
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