Metabolic conditions in diabetes alter the exhibition of insulin receptor in human tumoral T Cells
Abstract
Obese and diabetic population present higher frequencies of certain types of cáncer. This justifies the need for early-stage tumoral markers. The aim of this study was to test the effects of diabetic metabolic conditions on insulin receptor (IR) protein levels and exhibition in human tumoral T cells (Jurkat) by Western-blot and flow cytometry, respectively. Our results indicate that hyperglicemia doubles the total protein levels of the beta subunit of the receptor, while lowering the levels of the alpha subunit. High free fatty acids levels (FFA) reduces the levels of both subunits in hyperglicemia, but not in normoglicemia. Hyperglicemia lowers the exhibition of IR but high FFA concentrations in hyperglicemic conditions increases it by 60%. We conclude that diabetic metabolic conditions alter the functionality of the IR in Jurkat cells. This could link diabetes to tumoral proliferation.Downloads
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