Characterization of IgM, total IgG, IgG1 and Hcabs in colostra of llamas (Lama glama) by Elisa

  • Nicolás Caggiano Cátedra de Fisiología Animal, Facultad de Ciencias Veterinarias.
  • Natalia Saccodossi Cátedra de Inmunología Facultad de Farmacia y Bioquímica. Universidad de Buenos Aires. República Argentina
  • Teresa Gentile Cátedra de Inmunología Facultad de Farmacia y Bioquímica. Universidad de Buenos Aires. República Argentina
  • María Angelina Chiappe Barbará Cátedra de Fisiología Animal, Facultad de Ciencias Veterinarias
  • Juliana Leoni Cátedra de Inmunología Facultad de Farmacia y Bioquímica. Universidad de Buenos Aires. República Argentina
  • Emilio De Simone Cátedra de Fisiología Animal, Facultad de Ciencias Veterinarias
DOI: https://doi.org/10.5209/rev_RCCV.2014.v8.n2.46537
Keywords: Colostrum, llama, HCAbs, IgM

Abstract

Objectives: to determine the levels of IgM, total IgG, IgG1 and HCAbs (IgG2 and IgG3) in colostra of llamas and to evaluate the concentration of HCAbs in relation to total IgG and the conventional isotype IgG1.

Methods: Fifteen pregnant llamas were used in this study, which were milked within the first 24 hours after delivery. Sandwich ELISAs were designed for the quantification of IgM, total IgG and IgG1. The HCAbs concentration was calculated by difference between total IgG and IgG1 (HCAbs = total IgG – IgG1).

Results: The levels of Igs were found to be: IgM= 17.02 mg/ml (SD= 9.85), total IgG= 42.54mg/ml (SD=27.79), IgG1=24.34mg/ml (SD=13.96) and HCAbs (IgGtotal-IgG1) = 18.19 mg/ml (SD=15.49). Results regarding total IgG are consistent with those described in other species with similar type of placentation. HCAbs represented 43% of colostral IgG and IgM concentration presented values that were much higher (two and three times respectively) than those described for species with similar type of placentation.

Conclusions: This study is the first which evaluate amounts of each immunoglobulin isotype in colostra of llamas. We described a higher value of IgM in in comparison with species with similar placentation type. The IgG1 isotype, which represents the conventional isotype, is the more abundant antibody in colostra, being the HCAbs the second important fraction. This information may be useful in the management of neonates with failures in the transference of maternal passive immunity by colostra.

Downloads

Download data is not yet available.

Article download

Crossmark

Metrics

Published
2014-09-09
How to Cite
Caggiano N., Saccodossi N., Gentile T., Chiappe Barbará M. A., Leoni J. y De Simone E. (2014). Characterization of IgM, total IgG, IgG1 and Hcabs in colostra of llamas (Lama glama) by Elisa. Revista Complutense de Ciencias Veterinarias, 8(2), 29-40. https://doi.org/10.5209/rev_RCCV.2014.v8.n2.46537
Issue
Vol. 8 No. 2 (2014)
Section
Articles

License

In order to support the global exchange of knowledge, the journal Revista Complutense de Ciencias Veterinarias is allowing unrestricted access to its content as from its publication in this electronic edition, and as such it is an open-access journal. The originals published in this journal are the property of the Complutense University of Madrid and any reproduction thereof in full or in part must cite the source. All content is distributed under a Creative Commons Attribution 4.0 use and distribution licence (CC BY 4.0). This circumstance must be expressly stated in these terms where necessary. You can view the summary and the complete legal text of the licence.

Revista Complutense de Ciencias Veterinarias is an open access journal that does not charge authors for article processing (submission, review or editing) or publication.