Estandarización de una técnica de extracción de ADN en sementales porcinos para evaluar la frecuencia de los genes ESR y PRLR con PCR-RFLP

Resumen

In order to standardize extraction technique and purification of DNA from hair in swine, samples of 35 sires of Yorkshire and Landrace maternal Lines were obtained and the frequency of the genes ESR and PRLR were valued using PCR-RFLP. As a result of this technique a high quantity and quality of DNA was extracted. Two genes related with high prolificidad were identified: Estrogen (ESR) and prolactina (PRLR) receiver. These genes were amplified using PCR and RFLP´s and the genotypes were identified. The enzyme Pvu II was used to identification of ESR and Alu I for PRLR. The results showed that the genotypes associated to the genes ESR and PRLR were not in Hardy-Weinberg balance (X2,p<0.05). The favorable allele A of the gene PRLR and B of the gene ESR it showed frequencies of 0.21 and 0.14 respectively. There were not homocigote genotypes BB for the ESR gene.