Notas preliminares sobre las técnicas de amplificación y variación de la región ITS (Internal Transcribed Spacer) en el género Encalypta (Encalyptaceae, Bryophyta).

  • M.P. Martín
  • I. Álvaro
Keywords: Encalyptaceae, Musci, Iberian Peninsula, DNA Amplification, ITS

Abstract

MARTÍN M. P. & ÁLVARO I. 2001. Preliminar notes about the amplification techniques and the variation of the ITS region in the genus Encalypta (Encalyptaceae, Bryophyta). Bot. Complutensis 25: 233-239. Results on DNA amplifications are presented for the Encalypta species present in the Iberian Peninsula. DNA was isolated from the gametophyte and sporophyte, from both fresh and herbarium materials. DNA was also isolated from protonema obtained from cultures in vitro of spores. The internal transcribed spacers 1 and 2, including the 5.8S nrDNA 233 M.a Paz Martín & I. Álvaro Notas preliminares sobre las técnicas de amplificación... have been amplified. Pair primers ITS5/ITS4, ITS1/ITS4, ITS1/ITS2 and ITS3/ITS4 were tested by using two different amplification protocols. ITS5/ITS4 and ITS1/ITS4 yielded amplimer only from fresh samples, not from herbarium collections, from which only primers ITS1/ITS2 and ITS3/ITS4 amplified successfully each of the ITS regions separately.

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Published
2001-01-01
How to Cite
Martín M. y Álvaro I. (2001). Notas preliminares sobre las técnicas de amplificación y variación de la región ITS (Internal Transcribed Spacer) en el género Encalypta (Encalyptaceae, Bryophyta). Botanica Complutensis, 25, 233. https://revistas.ucm.es/index.php/BOCM/article/view/BOCM0101110233A
Issue
Vol. 25 (2001)
Section
Others

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